FIG. 4.

Effects of calcofluor white and hygromycin B on growth of the ΔAfpmt1 mutant. (Top) A series of 10-fold dilutions (10⁷ to 10³ cells) of A. fumigatus strains were spotted on solid complete medium containing 25 μg/ml of calcofluor white. Cells were incubated for 24 h at 37°C or 42°C. (Bottom) A series of 10-fold dilutions (10⁷ to 10³ cells) of A. fumigatus strains were inoculated on solid medium containing 25 μg/ml of hygromycin B and incubated at 37°C for 36 h. The wild-type strain YJ-407 (Afpmt⁺ pyrG⁺) and the parental strain of the mutant, CEA17 (Afpmt⁺ pyrG⁻), were set as controls for the ΔAfpmt1 mutant (Afpmt⁻ pyrG⁺) and the revertant (Afpmt⁺ pyrG⁻), respectively.