TABLE 1.
Primers used in this study
| Primer | Sequence (5′-3′)a | Restriction enzyme(s) |
|---|---|---|
| AFPMT1-5-N | GGTGGTGCGGCCGCCTTGCTTCCTCCTCAGGATCGA | NotI |
| AFPMT1-5-C | GGTGGTTCTAGACGACCGCAATCAATTAGGACCAAGT | XbaI |
| AFPMT1-3-N | GGTTCTAGATGAACGCCCGACGGCTGACG | XbaI |
| AFPMT1-3-C | GTGGTGAATTCGCGGCCGCCATTGGCACCCTTGCGCGCTC | EcoRI and NotI |
| AFPMT1-flank-5 | AAATATGCGGCCGCCTTGCTTCCTCCTCAGGATCG | |
| AFPMT1-flank-3 | GTCCGGAATTCGCATTGGCACCCTTGCGCGCTC | |
| N-flanking-5 | CCTGGTTGGCGGCTATCAGA | |
| C-flanking-3 | GCGTTGATGATGCAATTGGG |
a
Restriction enzyme sites are underlined.