TABLE 2.
In vitro O-mannosyltransferase activity of ΔAfpmt1 mutanta
| Strain | Activity (dpm/mg/h) | Relative activity (%) |
|---|---|---|
| Wild type | 4,907 ± 33 | 100 |
| ΔAfpmt1 | 1,969 ± 221 | 40 |
| Revertant | 5,257 ± 40 | 107 |
| CEA17 | 5,512 ± 532 | 112 |
a
Twenty-five micrograms of membrane protein fraction from the wild-type (YJ-407), ΔAfpmt1, revertant, or CEA17 strain was incubated for in vitro mannosyltransferase assay following the transfer of [3H]mannose from Dol-P-[3H]Man to the peptide Ac-YATAV-NH2 (see Materials and Methods). The assay was repeated at least three times. Average values (± standard deviations) from a typical experiment are shown.