TABLE 1.
Yeast strains used in this study
| Strain name | Description | Reference or source |
|---|---|---|
| THP17 | h90 ade6-M210 ura4-D18 leu1-32 | 28 |
| MI160 | h90 ade6-M210 ura4-D18 leu1-32 sst4Δ::ura4+ | See text |
| MI368 | h90 ade6-M210 ura4-D18 leu1-32 sst4Δ::kanMX6 | See text |
| MI597 | h90 ade6-M210 ura4-D18 leu1-32 sst4R193A | See text |
| MI578 | h90 ade6-M210 ura4-D18 leu1-32 sst4S271D | See text |
| MI550 | h90 ade6-M216 ura4-D18 leu1-32 sst4S316D | See text |
| MI577 | h90 ade6-M210 ura4-D18 leu1-32 sst4S271,316D | See text |
| MI545 | h90 ade6-M210 ura4-D18 leu1-32 hse1S176D | See text |
| MI596 | h90 ade6-M210 ura4-D18 leu1-32 sst4S271,316Dhse1S176D | See text |
| MI392 | h90 ade6-M210 ura4-D18 leu1-32 sst4Δ::kanMX6 hse1Δ::ura4+ | This studya |
| MI512 | h90 ade6-M210 ura4-D18 leu1-32 sst4+-mRFP5::kanMX6 | See text |
| MI446 | h90 ade6-M210 ura4-D18 leu1-32 sst4+-HA-FLAG::kanMX6 | See text |
| MI301 | h90 ade6-M210 ura4-D18 leu1-32 hse1Δ::ura4+ | See text |
| MI452 | h90 ade6-M210 ura4-D18 leu1-32 sst2Δ::ura4+ | See text |
| MI297 | h90 ade6-M210 ura4-D18 leu1-32 sst6Δ::ura4+ | See text |
| MI532 | h90 ade6-M210 ura4-D18 leu1-32 vps28Δ::ura4+ | See text |
| MI533 | h90 ade6-M210 ura4-D18 leu1-32 vps20Δ::ura4+ | See text |
| MI298 | h90 ade6-M210 ura4-D18 leu1-32 vps32Δ::ura4+ | See text |
| MI534 | h90 ade6-M210 ura4-D18 leu1-32 vps4Δ::ura4+ | See text |
| TW747 | h+/h−ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 | 41 |
| MI619 | h90/h90 ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 sst4Δ::ura4+/sst4Δ::ura4+ | This studyb |
| TP3 | h+/h−ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 pik3Δ::ura4+/pik3Δ::ura4+ | 28 |
| YS2 | h+/h−ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 ste12Δ::ura4+/ste12Δ::ura4+ | 42 |
a
This strain was constructed by crossing MI306 and MI368.
b
This strain was constructed by mating MI160 to an ade6-M216 segregant from a cross of MI160 to THP18 (h90 ade6-M210 ura4-D18 leu1-32).