FIG. 5.

Lack of fertility on M2 medium and M2 medium supplemented with methionine of strains lacking PaTrx1/PaTrx3 and overexpressing PaTrx2 (surT2 Δ1Δ3). mat+/mat− heterokaryotic strains of the indicated genotypes were incubated for 10 days on M2 medium or M2 medium supplemented with 20 μg of methionine/ml, at which time the wild type produces fully mature perithecia, whereas strains with PaTrx1 and PaTrx3 deleted do not produce mature fructifications even if PaTrx2 is overexpressed. A longer incubation time does not result in perithecium maturation in these mutants.