TABLE 1.

Effect of thioredoxin deletions during P. anserina life cycle

Category	Parameter	Straina
		WT	Δ1	Δ2	Δ3	Δ1Δ2	Δ1Δ3	Δ2Δ3	Δ1Δ2Δ3	surT2 Δ1Δ3
Methionine auxotrophy	Growth rate (mm/day) on medium lacking methionine	7	6	7	7	6	6	7	5	7
Vegetative growth on medium supplemented with methionine	Morphology	WT	WT	WT	WT	WT	Flat and dark	WT	Flat and dark	WT
	Longevity (mean cm ± SD)	9.3 ± 1.0	16.8 ± 3.7	8.6 ± 1.4	9.5 ± 1.2	>27	>27	12.0 ± 1.0	>27	NT
	CG on M2	No	No	No	No	No	No	No	No	No
	CG on M2 +YE	Yes	Yes	Yes	Yes	Yes	No	Yes	No	No
	H2O2 inhibition dose (%)b	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01	0.01
	H2O2 lethal dose (%)c	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05	0.05
Sexual reproduction	Perithecium production	Yes	Yes	Yes	Yes	Yes	No	Yes	No	No

^aWT, wild-type strain; Δ1, ΔPaTrx1 strain; Δ2, ΔPaTrx2 strain; Δ3, ΔPaTrx3 strain; YE, yeast extract; flat, fewer aerial hyphae than WT; dark, more pigmented than WT. NT, not tested.

^bThat is, the dose at which the growth rate of the indicated strains starts to decrease to 90% of that on medium not supplemented with H₂O₂. The same doses promote the same effects on medium lacking methionine or on medium supplemented with cysteine.

^cThat is, the dose at which the indicated strain does not present any sign of growth after 2 days of incubation.