FIG. 5.
Cytokine stimulation by Cacwh41Δ, Carot2Δ, and Camns1Δ null mutants. Human PBMC were stimulated 24 h with 106 yeast cells/ml and the TNF (A) and IL-6 and IL-10 (B) concentrations were determined. The experiments for panel A were carried out in the absence (□) or presence (▪) of laminarin, a blocking agent of the β-glucan/dectin-1 recognition pathway. The strains tested are NGY152 (wild type), HMY19 (Cacwh41Δ), HMY20 (Cacwh41Δ + CaCWH41), HMY12 (Carot2Δ), HMY13 (Carot2Δ + CaROT2), HMY5 (Camns1Δ), and HMY6 (Camns1Δ + CaMNS1). The results are pooled data from four volunteers. Error bars indicate the means ± the standard deviation. *, Significant differences in the mutant compared to the wild type (P < 0.05).