FIG. 3.
T cells infiltrate the ear during M. leprae infection. Mice were infected in the ear with 1 × 106 live M. leprae bacilli, and ears were collected at regular intervals to provide single-cell suspensions. Cell suspensions were subjected to flow cytometry. (A) Representative flow cytometry plots. Various cell types were enumerated as (B) total cells, (C) αβ T cells (TCRβ+), (D) CD4 T cells (CD4+, TCRβ+), (E) CD8 T cells (CD8+, TCRβ+), (F) dendritic cells (DC) (CD11c+), (G) macrophages (F4/80+), (H) granulocytes (Ly6G/C+), and (I) IFN-γ-producing cells (IFN-γ+ following PMA-ionomycin stimulation). Results are shown as the means and standard errors of the means for three mice per time (results for uninfected mice are plotted at week 0) and are representative of two or three similar experiments. *, P < 0.05; **, P < 0.01 (versus uninfected mice).