TABLE 1.
Primer | Nucleotide sequence (5′-3′) | Direction |
---|---|---|
SL283 | GAGGCGGGGCCCAGGCGGCCGAGCTC | Forward |
SL284 | GAGCCTGGCCGGCCTGGCCACTAGTG | Reverse |
SL371 | TATCTAGAGGCGTGGCGCTGCAA | Forward |
SL372 | TTGAAAGCTTTCACACGGCCGAATCGGC | Reverse |
SL373 | GTTCTAGACATCAGGGCGTGCTGG | Forward |
SL374 | AGCTAAGCTTTCAGCCTTTGGTGGCCATGA | Reverse |
SL390 | ATTCTAGAAAGGTACAGGCCACGCTGTTGAATG | Forward |
SL391 | AGATAAGCTTTCAGCGATTGGCGTGCAGCGAGTT | Reverse |
Underlined sequences in SL371, SL373, and SL390 are the XbaI restriction sites, while those in SL372, SL374, and SL391 are the HindIII restriction sites.