FIG. 6.
Synergistic effects of both overproducing LLO and increasing its in vivo half-life on the intracellular growth of L. monocytogenes in mouse cells. Graphs depict the intracellular growth, in J774 macrophages, of L. monocytogenes strains that overproduced LLO (owing to the LLOS44S mutation) and, when indicated, also contained mutational alterations of the N-terminal residue of LLO (K25X). See Materials and Methods for technical details. Error bars indicate standard deviations of results for triplicate coverslips from one experiment representative of three independent experiments.