TABLE 1.
Constructs and L. monocytogenes strains used for this study
| LLO proteina | LLO plasmidb | LLO plasmid L. monocytogenes strain in background:
|
|
|---|---|---|---|
| Δhlyc (DP-L2161) | ΔactA Δhly (DP-L4813) | ||
| Wild type | DP-E4817 | DP-L4818 | DP-L4896 |
| K25R | DP-E4861 | DP-L4928 | DP-L4882 |
| K25G | DP-E4874 | DP-L4929 | DP-L4899 |
| K25V | DP-E4875 | DP-L4930 | DP-L4900 |
| K25L | DP-E4876 | DP-L4931 | DP-L4901 |
| K25F | DP-E4995 | DP-L4997 | DP-L4996 |
| K25A | DP-E4880 | DP-L4933 | DP-L4905 |
| K25T | DP-E4879 | DP-L4934 | DP-L4903 |
| S44S | DP-E4878 | DP-L4966 | |
| K25R S44S | DP-E4912 | DP-L4967 | |
| K25G S44S | DP-E4913 | DP-L4968 | |
| K25V S44S | DP-E4914 | DP-L4969 | |
| K25L S44S | DP-E4915 | DP-L4970 | |
| K25F S44S | DP-E4921 | DP-L4971 | |
| K25A S44S | DP-E4961 | DP-L4978 | |
| K25T S44S | DP-E4962 | DP-L4979 | |
a
Mutant proteins are identified by their mutations.
b
DP-E numbers indicate pPL2-LLO constructs in E. coli.
c
Reference 32.