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. 2007 Aug 20;75(11):5095–5104. doi: 10.1128/IAI.00075-07

FIG. 3.

FIG. 3.

HEp-2 and LRP receptor binding ELISAs. (A) Indirect ELISA that served as an internal control for subsequent binding ELISAs. Purified toxins (5 μg/well) were used as coating antigens and detected with CNF1 polyclonal antisera at a dilution (1:5,000) that allowed comparable signals from all toxins assayed. The data are the averages of two experiments done in triplicate. (B) HEp-2 receptor binding ELISA in which purified toxins (5 μg/well) were added to fixed HEp-2 cells for 1 h and bound toxin was detected with anti-CNF1 polyclonal sera. The data are the averages of two experiments done in triplicate. (C) LRP binding ELISA in which purified toxins were incubated in LRP-coated ELISA plate wells and bound toxin was subsequently detected with anti-CNF1 polyclonal sera. The data are the averages of two experiments done in triplicate. In all panels, the error bars indicate the standard deviation above and below the mean. Also, the values for wells containing toxin but no anti-CNF1 sera served as background controls and were subtracted from all sample values.