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Complementation of the temperature-sensitive growth phenotype of E. coli lpxL. Cultures were grown at 30°C (A), 37°C (B), and 42°C (C) with agitation at 225 rpm. ⧫, strain B178, wild-type E. coli; •, strain MLK217, E. coli lpxL mutant; ▴, strain MKM10, E. coli lpxL mutant expressing F. tularensis FTT0232c; ▪, strain MKM11, E. coli lpxL mutant expressing F. tularensis FTT0231c. Absorbance was measured by the optical density at 600 nm (OD600).
