FIG. 5.

F. tularensis intracellular survival during infection of MDM. MDM monolayers were infected at an MOI of 10 bacteria to 1 macrophage. After 2 h of phagocytosis, extracellular bacteria were washed from the monolayers. Monolayers were disrupted 0, 4, and 10 h after removal of extracellular bacteria to enumerate intracellular bacteria by counting the number of CFU. Data are from three independent experiments, and standard errors of the means were used to derive error bars.