TABLE 2.
Oligonucleotides used in this studya
| Name and procedure | Nucleotide sequence (5′-3′) | Gene or target |
|---|---|---|
| PCR | ||
| MKM1 | CCAGTGCTAATGGGATTATCAAT | FTT0232C |
| MKM4 | GGTTCTCATAAGCGATATCATTA | FTT0232C |
| MKM5 | GGTAATGATCTAGAAGATGCGAA | FTT0231C |
| MKM6 | GGTATCTGGATTGTCGTTGGTTA | FTT0232C |
| MKM7 | GGTCTGGTTCTATAGCGACGAT | FTT0232C |
| MKM8 | GCCGCACTGGCTGCTGAA | FTT0231C |
| MKM82 | GTACCCCGGGGGAGCATACCACATAGAGAC | FTT0232C |
| MKM83 | CATGCCCGGGGGTATCTGGATTGTCGTTGG | FTT0232C |
| MKM85 | CAGTGGATCCGGCTGTTTGCCTATGCTTATACA | FTT0232C |
| MKM86 | CTGAGGATCCGGTATACCTATAGATGCAAGAATA | FTT0232C |
| MKM104b | GCACTCGACTGCAGGGATCTTCTTGAGATCCTTT | oriE |
| MKM105 | CCGAGCTACTCGAGTATACTTTGGCGTCACCCCT | sacB |
| MKM106 | GACTCTCGAGCTGCACGACGAACTAATACT | groEL promoter |
| MKM107 | CGTCCTCGAGCCGATTTAGAGCTTGACGG | F. tularensis ori |
| MKM128 | AGAATATCACCGGAATTGAAAAAAC | aphA-3 |
| MKM129 | TGATCCCCAGTAAGTCAAAAAATAG | aphA-3 |
| MKM130 | CATCTGCAGCGATTTTCGTTCGTGAATAC | aad(9) |
| MKM131 | CATCTCGAGAAGGTGTTTCCACCATTTTTTC | aad(9) |
| SPECRup | CGATTTTCGTTCGTGAATAC | aad(9) |
| SPECRdown | AAGGTGTTTCCACCATTTTTTC | aad(9) |
| M13 forward | GTAAAACGACGGCCAG | MCS, pCR2.1TOPO |
| M13 reverse | CAGGAAACAGCTATGAC | MCS, pCR2.1TOPO |
| RT-PCR | ||
| MKM21 | GCGTCCTTTCTATAGAATCGTA | 16S rRNA |
| MKM22 | GATTAAGCTTGCTACTCTATCAGA | 16S rRNA |
| MKM19 | GGTCGCTATATTGGTGAGAACTAT | FTT0232C |
| MKM20 | GGAGCATACCACATAGAGACTTT | FTT0232C |
| Real-time RT-PCR | ||
| MKM26 | GAAGAGAGATTAAAGCTTGATGTTGCT | 16S rRNA |
| MKM27 | GCTTGCTACTCTATCAGAAGGTTGTG | 16S rRNA |
| MKM36p | AAGCTGAAGCATGGTTAGCAAAAGGT | 16S rRNA |
| MKM28 | CAAAATGCTAATGCGTCTTGCA | FTT0232C |
| MKM29 | ATTGGTAATTGCTATTTGTTGACGTT | FTT0232C |
| MKM37p | AGGCATTGGTTTTTTAGCAAAGCCTCTACTCAAA | FTT0232C |
a
Restriction endonuclease sites are underlined. p, 5′-56-6-carboxyfluorescein, 3′-36-TAMTph-labeled TaqMan probe; MCS, multicloning site.