FIG. 5.

Western blot analysis of F. tularensis LVS or F. novicida U112 cell lysates with a mouse monoclonal anti-F. tularensis LPS antibody. The antibody recognizes LPS from F. tularensis LVS and Schu S4 but not LPS from F. novicida U112. Each HimarFT mutant strain is indicated by the gene in which HimarFT insertion was mapped, as shown in Table 2. Multiple strains with mutations in the same gene are indicated by the gene designation and a candidate number. The previously studied F. tularensis LVS ΔpurMCD mutant is indicated by MCD (53).