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. 2007 Sep 4;75(11):5509–5517. doi: 10.1128/IAI.00658-07

FIG. 3.

FIG. 3.

Comparison of DNA binding between CRPMt and CRPBCG, and the effects of cAMP on CRPBCG binding. (A) Binding of CRPMt and CRPBCG with the serC-Rv0885 intergenic region in the absence of cAMP. Lanes: 1, free probe; 2 to 6, CRPMt; 7 to 11, CRPBCG. Lanes 2 to 6 and 7 to 11 are in the presence of 0.35 nM, 0.7 nM, 1.7 nM, 3.5 nM, and 7 nM purified protein, respectively. (B) Comparison of CRPMt's and CRPBCG's binding with the serC-Rv0885 intergenic region in the presence of 100 μM cAMP. Probe and protein dilutions are the same as the lanes in panel A. (C) CRPBCG's binding with the serC-Rv0885 intergenic region in the absence (lanes 1 to 6) or in the presence (lanes 7 to 12) of cAMP. Lanes 1 and 7 are free probes. Lanes 2 to 6 and 8 to 12 are in the presence of 0.35 nM, 0.7 nM, 1.7 nM, 3.5 nM and 7 nM purified protein, respectively. DNA binding data are plotted to the right of each panel. For each panel, quantitation of band intensity was done using ImageQuant software, and the percent DNA bound was calculated as the intensity of each shifted band divided by the intensity of the unshifted band in the probe-only sample (lane 1). The apparent dissociation constant (kd) (37) in nM is also indicated.