TABLE 1.
Primers used in this study
| Assay type and gene namea | Primer | Oligonucleotide sequenceb |
|---|---|---|
| EMSA and ChIP | ||
| Rv3857c | KM1218 | F: GGATCCTAGCCAACCTGATACGTGCC |
| KM1219 | R: GGATCCGAGGGCACAGTTCATC | |
| serC-Rv0885 | KM1229 | F: GGATCCTATGCGCGTCCTGTCCATTC |
| KM1230 | R: GGATCCTGGTCGGCCATGCCATCAG | |
| frdA | KM1260 | F: GGATCCGTAAACACAATCGCAGAACTGG |
| KM1261 | R: GGATCCATAACCACGATGTTGTGTTGGG | |
| Rv0019c | KM1262 | F: GGATCCTCGTCCGCATGCACTGAACC |
| KM1263 | R: GGATCCGTCAGTTGCAGTACCAACC | |
| PE15 | KM1266 | F: GGATCCTATCTCGCTGCCGTGTAGC |
| KM1267 | R: GGATCCGGAACGACTCGCAACGTCAC | |
| Rv0145 | KM1270 | F: GGATCCACCTGACCGAGACCTAAC |
| KM1271 | R: GGATCCTCGCGAGGATGGTAACGAGG | |
| Rv0950c-sucC | KM1601 | F: GGATCCAACTCCTTGGCTTGATACTC |
| KM1602 | R: GGATCCGAGGTGTGCGAATCGCTG | |
| Rv1158c-Rv1159 | KM1641 | F: GGATCCTAGCGATCGCCGAGCGCACC |
| KM1642 | R: GGATCCGCGGACAAACGTCCAGATGG | |
| sigA internal fragment | KM1309 | F: GATGACCGAGCTTAGCGAGC |
| KM1310 | R: CGTAGGTGGAGAACTTGTACC | |
| RT-PCR | ||
| Rv3676 | KM859 | F: ACGGCCGAGAAAACCTGTTAACC |
| KM860 | R: GACCCGCAATGCGACCTTCC | |
| 16S rRNA | KM886 | F: GCGAACGGGTGAGTAACACG |
| KM887 | R: TGAAAGAGGTTTACAACCCG | |
| Rv0145 | KM1272 | F: ATAGCTGCCGGATTCGATGG |
| KM1273 | R: GGCTCGTGGTAGGTCAACG | |
| frdA | KM1274 | F: GCGATAGCCGAAACCAATCC |
| KM1275 | R: GTGAAATCCCGTCTTGTCGG | |
| Rv0019c | KM1276 | F: TCATCTGGTCCGTGCTACGG |
| KM1277 | R: ATCCTAGATCTTCGACGTACC | |
| PE15 | KM1280 | F: CTGCCATCGAAGCAGTGACC |
| KM1281 | R: CCACCGCTGAGATACGACGC | |
| Rv0885 | KM1282 | F: CGAGTACGTGGAAATGCTGG |
| KM1283 | R: GAACGTGTAGTTCATCAGGC | |
| serC | KM1286 | F: GGCTATGAGGTGATACTGGG |
| KM1287 | R: GGCGTCGATGACGACCAAGG | |
| sucC | KM1288 | F: TTCGCCAAGCACAACGTGCC |
| KM1289 | R: TATCGCTAGCCTCAGCGACC | |
| sigA | KM1309 | F: GATGACCGAGCTTAGCGAGC |
| KM1310 | R: CGTAGGTGGAGAACTTGTACC | |
| Rv1158c | KM1643 | F: GGTATCGCTCACGCAGATCC |
| KM1644 | R: CGTCAGTCCTGGAAAGGTGG | |
| Rv0950c | KM1675 | F: GTGCTGACCGCTCATGCATC |
| KM1676 | R: GCGTTAGCCAGGTCGATACC | |
| Rv1159 | KM1677 | F: CATTTTCGGTGGTCGTGTTCC |
| KM1678 | R: GCAGCAGGCCGAACAACTCG | |
| Rv3857c | KM1679 | F: CTCGGATTTGATACGAAACC |
| KM1680 | R: CAGTTGCCCGAAGACGACCC |
a
The target sequence for each gene is the intergenic region for EMSA and the ChIP assay and an internal region for RT-PCR, according to the complete sequence of M. tuberculosis H37Rv on the TubercuList World Wide Web server (http://genolist.pasteur.fr/TubercuList/).
b
Oligonucleotide sequences are from 5′ to 3′. F, forward primer; R, reverse primer.