TABLE 3.
Real-time PCR analysis of gene expression
| Genea | Microarray (relative fold induction)b
|
Real-time PCR (relative fold induction)
|
||||
|---|---|---|---|---|---|---|
| H. pylori positive/H. pylori negative | Triple negative/H. pylori negative | Triple positive/ H. pylori negative | H. pylori positive/H. pylori negative | Triple negative/H. pylori negative | Triple positive/ H. pylori negative | |
| ENA-78 | 12.3 | 6.9 | 15.6 | 23.6 | 15.0 | 27.8 |
| IL-8 | —c | —c | —c | 8.8 | 7.4 | 9.5 |
| C3 | 13.1 | 13.6 | 12.8 | 14.0 | 15.7 | 13.1 |
| LTF | 14.6 | 12.8 | 15.6 | 16.8 | 17.3 | 16.6 |
| TLR6 | 20.8 | 23.8 | 18.9 | 5.1 | 4.0 | 5.6 |
| IGFBP2 | 0.3 | 0.4 | 0.3 | 0.9 | 1.2 | 0.7 |
a
Abbreviations: LTF, lactotransferrin; IGFBP2, insulin-like growth factor binding protein 2.
b
Ten triple-positive and six triple-negative H. pylori-infected samples were compared with eight noninfected samples. The relative fold induction was calculated by dividing the average normalized gene expression in every H. pylori-infected group by the average normalized gene expression in the H. pylori-negative group.
c
IL-8 was not detectable using the microarray assay.