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. 2007 Aug 13;75(11):5453–5459. doi: 10.1128/IAI.00713-07

FIG. 4.

FIG. 4.

Identification of immunoreactive peptides in IOE OMP-19. The frequencies of IFN-γ-producing OMP-19 peptide-specific T cells in spleens collected from IOE OMP-19-immunized mice were determined by ELISPOT assay. Spleen cells from two immunized mice were pooled and cultured in duplicate in the presence of either OMP-19 (10 μg/ml) or a panel of overlapping 16-amino-acid IOE OMP-19 peptides. Data shown were obtained after subtraction of background; none of the peptides elicited responses from CD4 T cells obtained from unimmunized mice. Counts above the dotted line intercepting the value of 5 SFC/1 × 105 cells were considered significant. The sequences of the peptides are available in Table S1 in the supplemental material. SFC, spot-forming cells.