FIG. 1.

Growth and viability of B. abortus 2308 (diamonds), HR1703 (2308 bhuA) (squares), and HR1703RC (HR1703 bhuA⁺) (triangles) in (A) low-iron minimal medium and (B) low-iron minimal medium containing 50 μM FeCl₃. The data are means and standard deviations for triplicate determinations from a single flask for each strain at each experimental time point in a single experiment. An asterisk indicates that the P value is <0.05 for a comparison of the data obtained for HR1703 and the data obtained for 2308 and HR1703RC. The data are representative of multiple (≥3) experiments from which equivalent results and statistical trends were obtained.