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. 2007 Aug 27;75(11):5353–5360. doi: 10.1128/IAI.00922-07

FIG. 1.

FIG. 1.

UPEC isolate NU14 induces less urothelial CXCL-8 secretion than a piliated lab strain. (A) TEU-1 cultures were incubated with media only (Control), 1.5 ng/ml recombinant human TNF-α, or increasing amounts of either NU14 or the type 1 piliated lab strain HB101/p1-17. Cells were incubated with either bacterial strain at an MOI of 50:1, 100:1, 250:1, or 500:1. Supernatants were collected after 4 h, and CXCL-8 secretion was determined by ELISA. NU14 induced significantly less CXCL-8 secretion than HB101/p1-17 at all MOI. (B) TEU-1 cells were incubated with media alone (Control), 1.5 ng/ml TNF-α, NU14, or HB101/p1-17 for various times (MOI of 250:1), and CXCL-8 secretion was determined by ELISA. TNF-α and HB101/p1-17 elicited increased CXCL-8 secretion relative to control or NU14 at 3 or more hours of incubation. Data represent the means ± standard deviations of three separate infections, and each experiment was performed in duplicate. Asterisks indicate statistically significant differences between NU14 and HB101/p1-17.