FIG. 4.
Quantitation of myeloperoxidase (MPO) in mouse urine and bladder homogenates during experimental UTI by ELISA. Infection with NU14 results in less MPO in mouse bladder homogenates and urine than infection with MG1655. Mice were infected with 1 × 106 CFU NU14 or MG1655 via catheter (for bladder experiments, four control mice and nine infected mice; for urine experiments, 10 control mice, eight NU14-infected mice, and 10 MG1655-infected mice). Urine samples were collected, and the bladders were removed and homogenized 6 h postinfection. Bladder (A) and urine (B) MPO levels were determined by ELISA. Asterisks indicate statistically significant differences between uninfected controls and samples infected with strain MG1655.