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. 2007 Aug 13;75(11):5346–5352. doi: 10.1128/IAI.00689-07

FIG. 2.

FIG. 2.

Intracellular survival of Salmonella is enhanced in macrophages lacking ASM. The antimicrobial activities of macrophages from ASM+/+ and ASM−/− mice against wild-type (wt) (A) and spiC-deficient (B) Salmonella bacteria were monitored over time. Percent survival is expressed as the mean ± standard deviation (n = 6 to 10). Logarithmically transformed data were analyzed by two-way ANOVA. *, P < 0.05 for comparison of ASM+/+ and ASM−/− antimicrobial activities as determined by a Bonferroni posttest. (C) Internalization of bacteria by ASM+/+ and ASM−/− macrophages was estimated by recording the number of bacteria capable of forming a colony following 35 min of infection. Data are expressed as mean numbers of CFU ± SEM (n = 16). (D) Phagocytic indices were calculated for ASM+/+ and ASM−/− macrophages by enumerating the number of intracellular FITC-labeled Salmonella bacteria per macrophage by fluorescence microscopy. A total of 50 to 60 macrophages were analyzed from six separate slides per group. The numbers of bacteria per macrophage are represented in a box-and-whisker plot as median, intraquartile, and total ranges.