FIG. 4.

ASM-mediated anti-Salmonella activity of macrophages depends on a functional NADPH oxidase. (A) The effect that the flavoprotein inhibitor DPI has on the survival of wild-type (wt) and spiC-deficient Salmonella was studied in macrophages from ASM^+/+ and ASM^−/− mice. Data are expressed as mean percent survival ± standard deviation. (B) The effect of the NOS inhibitor MMLA on the survival of Salmonella in ASM^+/+ and ASM^−/− macrophages was determined after 20 h of infection. DPI and MMLA were added to the macrophages at the time of the infection. Data are expressed as mean percent survival ± SEM. (C) Mean ASM activity ± SEM was determined for untreated or DPI-treated ASM^+/+ macrophages (mφ) prior to (0 min) or 30 min following challenge with Salmonella. The data are from four to seven independent observations.