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. 2007 Oct 8;75(12):5678–5685. doi: 10.1128/IAI.00702-07

FIG. 5.

FIG. 5.

Effect of growth phase and expression of LspA1 and LspA2 on H. ducreyi uptake by and Src kinase activation in DC. (A) Percentages of DC containing GFP-expressing bacteria by flow cytometry after incubation with mid-log- and stationary-phase 35000HP/pRB157K or 35000HPΩ12/pRB157K for 90 min. Values are the means ± SDs of the percentages of GFP-positive DC obtained from seven or six donors and cultured with mid-log-phase (MOI of 10) or stationary-phase (MOI of 50 to 100) bacteria, respectively. (B) Western blot of DC incubated with mid-log- or stationary-phase 35000HP or 35000HPΩ12 for 4 h under the conditions described for panel A. Active forms of Src kinases were determined by the α-pY418 antibody. Actin levels were measured as loading controls. Similar results were observed for DC obtained from five donors.