TABLE 1.
Effect of H. ducreyi infection on activation of human monocyte-derived DC
| Treatmenta | Mean geometric MFI (SD) of surface marker expressionb
|
||||
|---|---|---|---|---|---|
| HLA-DR | CD40 | CD80 | CD83 | CD86 | |
| Medium alone | 134 (45) | 29 (3) | 17 (6) | 4 (1) | 37 (8) |
| Live H. ducreyi | 202 (32) | 83 (18) | 102 (43) | 15 (4) | 168 (61) |
| HK H. ducreyi | 374 (77)* | 160 (41)* | 257 (71)* | 38 (15)* | 454 (121)* |
| MC | 350 (104)* | 112 (33)* | 135 (43)* | 36 (18)* | 448 (148)* |
| E. coli LPS | 335 (80)* | 117 (37)* | 178 (54)* | 36 (16)* | 351 (97)* |
| Live H. ducreyi + LPS | 199 (37)** | 79 (18) | 93 (30) | 14 (3) | 154 (46)** |
| HK H. ducreyi + LPS | 389 (77)* | 154 (55)* | 271 (88)* | 42 (17)* | 504 (153)* |
a
DC were cultured for 48 h with medium alone, live H. ducreyi, heat-killed (HK) H. ducreyi, the maturation cocktail (MC), E. coli LPS, or a combination of live or heat-killed bacteria plus LPS.
b
MFI, mean fluorescence intensity. Values represent data for six donors. *, significant difference compared to medium alone (P < 0.05); **, significant difference compared to E. coli LPS alone (P < 0.05).