FIG. 2.

Dose-dependent reduction in C. trachomatis-induced IL-8 by an inhibitor of the ERK pathway. (A) Increasing concentrations of the MEK1/2 inhibitor of the ERK MAPK pathway were added 90 min prior to total RNA harvest at 24 hpi. The relative change in IL-8 mRNA was determined as described in the legend to Fig. 1. Data shown are averages ± SD. Data are from one of two experiments. **, P = 0.012; *, P < 0.01 (compared to infected cells without inhibitor treatment). (B) C. trachomatis-infected cells were treated with 2 μM p38 inhibitor or 1 μM JNK inhibitor at 24 hpi. One nanogram (p38) or 4 ng (JNK) IL-1α was added for 20 min prior to harvest at 25.5 hpi. Data are from one of two experiments.