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. 2007 Oct 1;75(12):5985–5992. doi: 10.1128/IAI.00678-07

FIG. 2.

FIG. 2.

P. aeruginosa activates p38 MAPK via flagellin protein in HAECs. (A) Effect of PAK and PAK/fliC on the activation of p38 MAPK. HAECs cultured in 24-well plates were incubated with heat-killed PAK or PAK/fliC at an MOI of 100 for the time intervals indicated. Phosphorylated and total p38 MAPK were analyzed by Western blotting. (B) Densitometric quantification of the results shown in panel A. Densitometry of the phosphorylated p38 MAPK was normalized by using the total loading control. The data show the fold increases for a comparison with the PBS control using normalized densitometry. (C) Effect of B. cepacia and B. cepacia BC/fliC on p38 MAPK activation. HAECs cultured in 24-well plates were incubated with heat-killed B. cepacia or B. cepacia BC/fliC (MOI of 100). Phosphorylated and total p38 MAPK were analyzed by Western blotting. (D) Densitometric quantification of the results shown in panel C. The data show the fold increases for a comparison with the PBS control using normalized densitometry. All images are representative, and the values are means and standard deviations from three independent experiments. Two asterisks, P < 0.01 for a comparison with the control; one asterisk, P < 0.05 for a comparison with the control.