FIG. 3.

C. trachomatis-specific ezrin Tyr-P is sustained to the mid-cycle of infection. (A) Ezrin Thr-P occurs independently of the infecting chlamydial strain. Cells remained uninfected or were infected with either C. trachomatis (D or L2) or GPIC (MOI of ∼0.5). Samples were harvested at the indicated times p.i. in Laemmli sample buffer, and Thr-P was determined by immunoblotting with an anti-phosphoERM Ab specific to the C-terminal Thr567. The same blots were stripped and reprobed for total ezrin. (B) Densitometry was performed on the blots shown in panel A. PhosphoERM levels were normalized to total ezrin levels within each sample. The graph shows the fold change in phosphoERM levels relative to uninfected cells, with values from uninfected cells set to 1. (C) Cells were infected as described above (MOI of ∼5), and ezrin was immunoprecipitated with an anti-rabbit ezrin Ab. The Tyr-P status was examined by Western blotting with the 4G10 Ab. The same blot was stripped and reprobed with the anti-ezrin Ab.