FIG. 5.

Ezrin functions during C. trachomatis entry. Control or DN LLC-PK1 cells were infected with C. trachomatis D or GPIC (MOI of ∼0.01). Cells were fixed at 2 h p.i., and extracellular bacteria were stained with a MOMP-specific Ab. The cells were permeabilized, and the total bacteria were stained with an LPS-specific Ab. The number of internalized EBs was quantified and categorized according to number of intracellular EBs per cell. Only with C. trachomatis D infection was there a significant reduction in the number of EBs per infected cell. *, P < 0.002.