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. 2007 Sep 24;75(12):5565–5574. doi: 10.1128/IAI.00405-07

FIG. 2.

FIG. 2.

Western blot analysis of SERA4-null parasites. (A) Western blots of total material from synchronized late-blood-stage parasites comparing the parental D10 wild type with two clones of D10-ΔSERA4 were probed with either rabbit anti-Hsp70 polyclonal or rabbit anti-SERA4 polyclonal antibody. The antisera used to probe each membrane are indicated at the bottom of the gel. Molecular mass markers (kDa) are shown to the left of the membrane. (B and C) Western blots of total material from synchronized late-blood-stage parasites from wild-type D10 (B) or D10-ΔSERA4 (clone 1) (C) probed with rabbit anti-SERA1 to anti-SERA9 polyclonal antibodies specific to the N-terminal region of each SERA protein (lanes 1 to 9, respectively). The antiserum used to probe each membrane strip is indicated at the top of the lane. The strip probed with the prebleed pool is indicated by P. Molecular mass markers (kDa) are shown to the left of each membrane.