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. 2007 Sep 24;75(12):5575–5585. doi: 10.1128/IAI.00443-07

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FIG. 5. — Immunoblot analysis of culture supernatants precipitated with TCA and detected with anti-LpnE, anti-Lpg1905, and anti-RpoB antibodies. (A) Stationary-phase TCA-precipitated culture supernatants and whole-cell lysate samples from derivatives of L. pneumophila 130b were separated by SDS-polyacrylamide gel electrophoresis. Lane 1, L. pneumophila 130b; lane 2, lpnE::km mutant; lane 3, lpnE::km/(pMIP:lpnE) mutant; lane 4, ΔdotA mutant; lane 5, ΔlspDE mutant. (B) TCA-precipitated stationary-phase culture supernatants. Lane 1, L. pneumophila lpnE::km/(pMIP:lpnE) mutant; lane 2, lpnE::km/(pMIP:lpnE_1-51) mutant; lane 3, lpnE::km/(pMIP:lpnE_1-122) mutant; lane 4, lpnE::km/(pMIP:lpnE_1-266) mutant; lane 5, lpnE::km/(pMIP:lpnE_52-375) mutant. The presence of both LpnE_1-266 and LpnE_52-375 in the culture supernatant is indicated by arrows and the predicted molecular size of each protein.