FIG. 2.

Antibody avidity does not differ significantly between F+C/M+C- and C/C-immunized mice. BALB/c mice were immunized with the C/C or F+C/M+C regimen. The interval between prime and boost was 2 weeks. Two weeks after boosting (day 28), blood was collected from the tail vein, sera were diluted in PBS to similar concentrations, and an avidity ELISA was performed. The rate at which DP4-specific antibodies were dissociated from the DP4 peptide by sodium thiocyanate was used as a measure of antibody avidity. Results are from one experiment and are expressed as the percentages of antibody bound at various NaSCN concentrations. Error bars represent ±95% confidence interval; n = 10.