FIG. 10.

(A) i.n. MIP-2 administration results in recruitment of neutrophils to bronchoalveolar spaces. Groups of C57BL/6 mice were instilled by the i.n. route with either 1.0 μg of recombinant murine MIP-2 (open bars) or 0.1% BSA diluent (solid bars). The mice were killed 24 h later, their lungs were lavaged, and total and differential cell counts were determined. The data are presented as mean ± SD (n = 5). ***, P < 0.005 versus mice treated with diluent. (B) Effect of i.n. MIP-2 administration on A. baumannii burden in the lungs. Groups of C57BL/6 mice were treated i.n. with either 2.5 μg of recombinant murine MIP-2 (open bars) or diluent (solid bars) in 50 μl at 24 and 4 h before i.n. challenge with 2 × 10⁷ CFU A. baumannii. At 24 h, the mice were killed, lung homogenates were prepared from individual mice, and CFU were determined. The data are compiled from three independent experiments with similar results; each individual dot represents the value from a single mouse, and the horizontal line represents the median value for the group (n = 15). **, P < 0.01 versus diluent-treated mice.