FIG. 11.

Effect of neutralization of endogenous TNF-α or IFN-γ on bacterial burdens in the lung following i.n. inoculation with A. baumannii. Groups of five C57BL/6 mice were treated by i.p. injection with either 500 μg of IFN-γ neutralization MAb R46A2 or TNF-α neutralization MAb 2E2 or an equivalent amount of control rIgG at 18 h before i.n. challenge with 2.0 × 10⁷ CFU of A. baumannii. Bacterial burdens in the lungs and spleens were determined by quantitative bacteriology. The data are presented as mean ± SD (n = 5), and the detection limit for the bacterial burdens was 1.3 log₁₀ CFU/lung.