FIG. 1.

O. volvulus-induced keratitis in TLR2^−/−, TLR4^−/−, and TLR2/4^−/− mice. OvAg was injected into the corneal stromata of C57BL/6, TLR2^−/−, TLR4^−/−, and TLR2/4^−/− mice. After 18 h, mice were sacrificed and corneas were examined as described in the text. (A) Single-cell suspensions were prepared from the corneas, and total neutrophils were detected by flow cytometry using the monoclonal antibody NIMP-R14. (B) Five-micrometer-thick corneal sections were immunostained using the same antibody, and cells were counted directly. (C) Corneas were examined by in vivo confocal microscopy. Values were exported to Excel and graphed, and the area under the curve was used to represent corneal haze. Statistically significant differences were seen between OvAg-injected C57BL/6, TLR2^−/−, and TLR2/4^−/− mice (P < 0.0001). Data points represent individual corneas, and the experiment is representative of four repeat experiments.