FIG. 3.

CXC chemokine production by corneal fibroblasts and whole corneas. (A and B) Corneas from C57BL/6 and TLR2^−/− mice were injected with 4 μg OvAg or HBSS (4 μl). After 6 h, corneas were dissected and sonicated, and CXCL1/KC and CXCL2/MIP-2 were measured by ELISA. (C) Corneal fibroblasts (MK-T cells) were incubated with 2, 10, or 50 μg/ml OvAg, with Wolbachia bacteria, or with 200 ng/ml Pam3Cys or LPS, and CXCL1/KC in supernatants was measured after 4 h of incubation. Results are shown as means plus standard errors of the means. The experiment was repeated twice with similar results.