FIG. 3.

Chemokine response patterns during early T. gondii infection or LPS stimulation of wild-type and MyD88 knockout macrophages. Bone marrow-derived macrophages were stimulated with LPS (100 ng/ml) or infected with type I (RH), type II (PTG), or type III (M7741) tachyzoites, and samples were collected at 6 h postinfection for analysis. A 6:1 ratio of parasites to cells was used, yielding >80% infection. (A) Response patterns of 114 chemokine, chemokine receptor, and related genes. Values are expressed relative to those for nonstimulated cells. The horizontal line defines 3 standard deviations from the mean for all genes for the sample showing the least amount of change (type III infection of MyD88^−/− macrophages). (B) Identities of genes upregulated by parasites and LPS. Numbers in parentheses indicate x-fold increases relative to levels in nonstimulated cells.