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. 2007 Oct 8;75(12):5867–5876. doi: 10.1128/IAI.00559-07

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FIG. 5. — ZnuA accumulation in intracellular salmonellae. (A) ZnuA accumulation in bacteria grown in rich medium (LB) and under zinc-limiting conditions (MM) compared to ZnuA accumulation in bacteria extracted from infected macrophages (J774), differentiated monocytes (THP-1), and colon epithelial cells (Caco-2). Each gel shows the results from two independent experiments. (B) Accumulation of ZnuA in bacteria harvested from spleen homogenates of BALB/c mice infected with strain SA140 (znuA::3xFLAG cat::3xFLAG) (lanes 1 and 2) or strain MA7225 (sodCI::3xFLAG cat::3xFLAG) (lanes 3 and 4). Each lane shows the epitope-flagged proteins recovered from a different mouse.