FIG. 1.

Analysis of DC maturation in response to infection with rough or smooth Brucella suis. (A) Immature human DCs were infected with B. suis WT, B. suis manB mutant, or E. coli and stained at 48 h p.i. for maturation marker expression. For each surface molecule studied, a cytometry analysis histogram from one representative experiment is presented on the left, and the compilation histogram on the right includes results (means ± standard errors of the means) from 21 independent experiments performed on cells from 21 distinct donors (**, P < 0.01, and ***, P < 0.001, versus B. suis WT-infected DCs, computed by Wilcoxon rank tests for CCR7 and CD83 or by paired Student t tests for other markers). (B) Immature DCs were infected with B. abortus 2308 and B. abortus 45/20 and stained at 48 h p.i. for maturation marker expression. Histograms include results from three independent experiments performed with cells from three distinct donors.