TABLE 1.

Intranasal rCPAF+IL-12 vaccination induces robust cell-mediated immune responses^a

Immunization	Cellular recall IFN-γ (pg/ml) productionb (mean ± SD) upon in vitro stimulation with:
	rCPAF	rMOMP	rIncA
PBS (mock)	<32	<32	<32
IL-12	<32	<32	<32
rCPAF+IL-12	1,525 ± 172	87 ± 34	66 ± 24
rMOMP+IL-12	64 ± 31	228 ± 72	96 ± 45
rIncA+IL-12	58 ± 26	90 ± 32	972 ± 128
C. muridarum	295 ± 95	155 ± 45	270 ± 80
HEL+IL-12	65 ± 30	52 ± 21	35 ± 13

^aAnimals (three mice/group) were immunized i.n. with rCPAF+IL-12, rMOMP+IL-12, rIncA+IL-12, C. muridarum (10⁵ IFU), the unrelated antigen HEL+IL-12, IL-12 alone, or PBS (mock immunized) alone. On day 14, the animals were euthanized, and splenic CD4⁺ T cells were purified and tested for antigen-specific IFN-γ production by ELISAs.

^bResults are representative of two independent experiments. The cellular recall IFN-γ production values were <32 for cells from animals immunized by all the immunization regimens shown after the cells were stimulated in vitro with medium.