TABLE 3.

Percentage of immunized animals shedding Chlamydia after genital challenge^a

Immunization	% Mice shedding Chlamydia from the vagina on the indicated day after i.vag. challenge
	3	6	9	12	15	18	21	24	27	30
PBS (mock)	100	100	100	100	100	100	50	50	33	0
rCPAF+IL-12	100	100	100	67	33	0	0	0	0	0
rMOMP+IL-12	100	100	100	100	100	67	50	17	0	0
rIncA+IL-12	100	100	100	100	83	50	33	17	0	0
rCPAF+rMOMP+IL-12	100	100	100	67	50	0	0	0	0	0
rCPAF+rIncA+IL-12	100	100	100	50	17	0	0	0	0	0
rCPAF+rMOMP+rIncA+IL-12	100	100	100	83	67	0	0	0	0	0
HEL+IL-12	100	100	100	100	100	100	67	50	33	0

^aAnimals (six mice/group) were immunized with three doses of rCPAF+IL-12, rMOMP+IL-12, rIncA+IL-12, rCPAF+rMOMP+IL-12, rCPAF+rIncA+IL-12, rCPAF+rMOMP+rIncA+IL-12, HEL+IL-12, or PBS (mock immunized). One month after the final booster vaccination, mice were challenged i.vag. with 10⁵ IFU of C. muridarum. Chlamydial shedding was measured at the indicated days following challenge. The time to resolution differed significantly between the experimental groups (P = 0.0002 by the Kaplan-Meier test). Specifically, rCPAF+IL-12, rCPAF+rMOMP+IL-12, rCPAF+rIncA+IL-12, and rCPAF+rMOMP+rIncA+IL-12, each demonstrated significantly earlier resolution, but not rMOMP+IL-12 or rIncA+IL-12, compared to PBS (mock) or HEL+IL-12 (p < 0.05). Results are representative of two independent experiments.