TABLE 1.

Results for selected patient sera tested by INKV IFA, protein-specific IgG IFA, and neutralization test^a

Patient no. or specimen type	IFA result				Protein-specific IgG IFA titer		Neutralization titer
	IgG titer	IgG pattern		IgM result
		g	d		N	Gc	INKV	SSHV	TAHV
1	160	+	−	Pos	120	<20	160	80	80
2	320	+	−	Pos	160	20	320	<20	<20
3	40	+	−	Pos	20	<20	160	20	40
4	40	+	−	Pos	ND	ND	320	<10	<20
5	160	+	−	Pos	ND	ND	160	20	20
6	80	+	−	Pos	<20	<20	160	20	20
7	320	+	−	Pos	160	<20	160	40	20
8	320	+	−	Pos	160	60	ND	ND	ND
9	≥80	+	−	Pos	20	<20	640	ND	ND
10	120	−	+	Neg	40	40	160	80	40
11	120	−	+	Neg	<20	40	160	80	80
12	240	−	+	Neg	<20	<20	320	<20	20
13	80	−	+	Neg	<20	<20	200	40	20
14	120	−	+	Neg	<20	40	220	160	160
15	40	−	+	Neg	40	80	200	20	20
16	60	−	+	Neg	20	80	200	60	40
17	160	−	+	Neg	<20	20	320	80	40
18	320	−	+	Neg	<20	160	640	ND	ND
MHAF	640	−	+	Neg	ND	ND	≥1,280	≥1,280	≥1,280

^aResults are from using INKV-infected Vero E6 cells and Sf-9 insect cells expressing individual INKV proteins as antigens and by neutralization test with INKV, SSHV, and TAHV. The N-specific titer difference between acute (patients 1 to 9) and long-standing immune responses (patients 10 to 18) was significant (P was 0.02, t was 2.8, and df was 6.5 by Student's t test for independent samples; the mean ± standard deviation for acute cases was 91.4 ± 74.7, and that for long-standing-immunity cases was 11.1 ± 17.6), but for Gc-specific titers, the difference was not statistically significant (P was 0.06, t was −2.1, df was 11.7, the mean ± SD for acute cases was 11.4 ± 22.7, and that for long-standing-immunity cases was 51.1 ± 50.1). Equal variances were not assumed. +, with indicated pattern; −, without indicate pattern; g, granular; d, diffuse; Neg, negative; Pos, positive; ND, not determined.