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. 1979 Jul;139(1):137–140. doi: 10.1128/jb.139.1.137-140.1979

Transduction of Pseudomonas aeruginosa with a mutant of bacteriophage E79.

A F Morgan
PMCID: PMC216838  PMID: 110777

Abstract

A mutant of the virulent bacteriophage E79 was isolated which mediated generalized transduction in Pseudomonas aeruginosa. Variable recovery of transductants as a result of phage killing was avoided by the use of recipients carrying the IncP-2 plasmid R38, and transduction frequencies of 4 X 10(-6) to 1 X 10(-5) per plaque-forming unit were obtained. Linkage studies have indicated that the coinheritance frequencies are less than would be expected from the published molecular weight of E79 deoxyribonucleic acid (120 X 10(6). By using recipients carrying R38, low-frequency transduction by wild-type E79 and two other virulent phages, F8 and phi 16, was demonstrated.

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Selected References

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