Detection of antibody to N. caninum by ELISA with NcSAG1 (A), NcSRS2 (B), NcGRA7 (C), and the parasite lysates (NLA) (D). Group 1 includes serum samples from aborting cows. Group 2 includes samples from nonaborting cows. Group 3 includes samples from heifers. The mean OD415 values are shown. Data were analyzed by analysis of variance, and the differences among the mean OD415 values were then analyzed using Turkey-Kramer multiple-comparison tests. *, statistically significant difference among the samples (P < 0.05). The OD415 values were representative of at least three repeated experiments.