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. 2007 Dec 20;27(2):406–420. doi: 10.1038/sj.emboj.7601967

Figure 6.

Figure 6

Effects of H3K36me3 knockdown on gene expression levels: lack of intragenic transcription from IE genes or gapdh. (A) C3H 10T½ cells were untransfected (−), mock transfected (no siRNA, mock) or transfected with Setd2, non-targeting (non-t) or NSD1 siRNA. Cells were quiesced 24 h later, and after a further 24 h left untreated (con) or treated with EGF (50 ng/ml) for 15–120 min. Total RNA was isolated and relative levels of Setd2 and NSD1 mRNA were quantified by qRT–PCR and normalised to 18S rRNA. Average values and s.d. from two independent experiments are plotted. (B) Cells were transfected and stimulated and total RNA isolated as in (A). Kinetics of c-fos, c-jun, CPBP and MKP1 gene expression was assessed by qRT–PCR and normalised to 18S rRNA. Average values and s.d. from two independent experiments are plotted. (C) Cells were transfected as in (A), left unstimulated and levels of cycb, polr3b, glnrs and gapdh mRNA quantified by qRT–PCR, with normalisation to 18S rRNA. Average values and s.d. from two independent experiments are plotted.