Figure 2.

Effect of incubation time of spleen homogenate to decrease nitrated BSA. (A) Nitrated BSA was incubated for the designated times (0–20 min) with spleen homogenate from rats treated with LPS. Spleen homogenate was also boiled (B) before incubation for 0–20 min with nitrated BSA, as designated. Nitrated BSA (N-B) was also incubated without homogenate as a control. This is a representative experiment with six different preparations. (B) Coomassie brilliant blue staining of a transferred membrane. Lanes are the same as in A. (C) Time-dependent denitration of nitrated BSA with spleen homogenates from six different preparations representing six rats. Some rats were treated with LPS (solid symbols) while others (open symbols) received vehicle as controls.