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. 2007 Sep 7;6(11):2139–2146. doi: 10.1128/EC.00174-07

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Copyright © 2007, American Society for Microbiology

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FIG. 1. — EhSTIRP gene family structure and expression. (A) The EhSTIRP gene family is made up of five members. The GenBank accession number is listed for each gene. EhSTIRP4 and EhSTIRP5 may represent truncated versions of the gene. The thinner bars at either end of the gene represent the 5′ promoter region and presumptive 3′ untranslated region. EhSTIRP2 and EhSTIRP3 have very similar 5′ promoter regions (98% nucleotide identity), whereas the 5′ promoter region from EhSTIRP1 was only 45% and 47% identical to EhSTIRP3 and EhSTIRP2, respectively. The gene family members are highly similar at both the nucleotide and amino acid levels towards the 3′ end/C terminus (∼99%) and less similar to one another at the 5′ end/N terminus. The overall similarity is higher between EhSTIRP2 and EhSTIRP3 than between either gene and EhSTIRP1. A signal peptide is shown for the three full-length gene family members and a transmembrane domain is shown for each gene. Gene-specific oligonucleotide probes are denoted as follows: . A PCR-generated probe capable of detecting all full-length EhSTIRPs and used in the dsRNA constructs is denoted as follows: . (B) E. histolytica HM-1:IMSS expresses each of the EhSTIRP full-length members, whereas E. histolytica Rahman does not express any of the full-length gene family members. Separate blots were probed for EhSTIRP expression in E. histolytica HM-1:IMSS, whereas since no signal was observed in E. histolytica Rahman, the same blot was sequentially probed. rRNA stained with methylene blue is shown as a loading control.

Inline graphic — EhSTIRP gene family structure and expression. (A) The EhSTIRP gene family is made up of five members. The GenBank accession number is listed for each gene. EhSTIRP4 and EhSTIRP5 may represent truncated versions of the gene. The thinner bars at either end of the gene represent the 5′ promoter region and presumptive 3′ untranslated region. EhSTIRP2 and EhSTIRP3 have very similar 5′ promoter regions (98% nucleotide identity), whereas the 5′ promoter region from EhSTIRP1 was only 45% and 47% identical to EhSTIRP3 and EhSTIRP2, respectively. The gene family members are highly similar at both the nucleotide and amino acid levels towards the 3′ end/C terminus (∼99%) and less similar to one another at the 5′ end/N terminus. The overall similarity is higher between EhSTIRP2 and EhSTIRP3 than between either gene and EhSTIRP1. A signal peptide is shown for the three full-length gene family members and a transmembrane domain is shown for each gene. Gene-specific oligonucleotide probes are denoted as follows: . A PCR-generated probe capable of detecting all full-length EhSTIRPs and used in the dsRNA constructs is denoted as follows: . (B) E. histolytica HM-1:IMSS expresses each of the EhSTIRP full-length members, whereas E. histolytica Rahman does not express any of the full-length gene family members. Separate blots were probed for EhSTIRP expression in E. histolytica HM-1:IMSS, whereas since no signal was observed in E. histolytica Rahman, the same blot was sequentially probed. rRNA stained with methylene blue is shown as a loading control.