FIG. 2.

Constructs used in analysis of EhSTIRP. Two different versions of the pJST4-EhSTIRP vector were created. The dsRNA construct contained two identical 395-bp segments in opposing orientations capable of producing dsRNA upon transcription. The 395-bp segment was amplified from the 3′ end of EhSTIRP in a region of high similarity among all family members and would be predicted to down-regulate the entire EhSTIRP gene family. The control construct contained two different regions of EhSTIRP1 and was incapable of forming dsRNA. Both the insert and the selectable marker Neo were placed under the control of the EhActin1 5′ and 3′ regulatory regions.